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Free t (equilibrium ultrafiltration). Once equilibrium is achieved, the free testosterone is separated from the bound testosterone by filtration through a membrane by centrifugal ultrafiltration. The current methods for the determination of free testosterone - equilibrium dialysis, ultrafiltration, tracer analog methods, and the use of algorithms. At equilibrium, this ratio is taken as a measure of the free testosterone fraction. Membrane ultrafiltration, and steady-state gel filtration. 1 free testosterone reference methods: equilibrium dialysis and ultrafiltration. 2 analog immunoassay methods for free testosterone. Overall, the results of equilibrium dialysis and centrifugal ultrafiltration methods have been. - 2014 annual tests - 21,000. Many methodology papers have been published concerning the measurement of free testosterone by ultrafiltration to which the reader is referred for specific. Free testosterone was calculated using vermeulen's formula. Testosterone with a candidate reference measurement procedure based on ultrafiltration and. A lesser fraction is albumin bound and a small proportion exists as free hormone. Historically, only free testosterone was thought to be the biologically. The equilibrium ultrafiltration test measures the percentage of free testosterone in a sample and uses that to calculate the free testosterone level. The ratio of free to protein-bound thyroxine (ftf) in human serum was measured by ultrafiltration of serum to which 131i-thyroxine was added

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